Samples from healthy dogs yielded negative results with all immunodiagnostic tests. Samples were also subjected to different DATs: a gel minitube and an immunochromatographic strip kit used in clinics neutral gel column cards, microtiter plates (at 4°, 22°, and 37☌), capillary tubes, and flow cytometry used in laboratories. Material and methodsĪnticoagulated blood samples from 126 dogs suspected of having IMHA submitted to a diagnostic veterinary laboratory for a routine direct antiglobulin test (DAT) and from 28 healthy control dogs were evaluated for spherocytosis and autoagglutination before and after three saline washes. As data on the performance of immunohematological tests was lacking, we undertook a comparative analysis. ![]() A 2019 ACVIM consensus statement on diagnostics for immune-mediated hemolytic anemia (IMHA) in dogs made testing recommendations.
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